Western Blotting Analysis: Representative lot data. HeLa nuclear extract was probed with JMJD8 (1ug/ml). Proteins were visualized using a Donkey Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates JMJD8 (~37kD). An uncharacterized band may be observed at ~160kD in some cell lysates.